SAMPLE COLLECTION OF THE EQUINE CONCEPTUS VIA VAGINAL FORNIX: STUDY OF THE DIAMETER AND LENGTH OF THE NEEDLE IN THE EFFICIENCY OF THE PROCEDURE
DOI:
https://doi.org/10.56238/revgeov17n3-204Keywords:
Conceptus, Equinus, Puncture, Transvaginal RouteAbstract
The determination of the sex of the embryo is of great zootechnical importance and has been improved over time. In humans, amniocentesis is performed successfully, and the samples obtained make it possible to perform genotyping and sexing of fetuses with precision. In order to perform the sexing and genotyping of equine embryos, in vivo, the present study aimed to test seven different needle gauges to obtain samples of yolk fluid and/or allantoic fluid from embryos and/or fetuses through transvaginal puncture. A total of 65 puncture procedures were performed, regardless of gestation age, in 29 mares, and 36 procedures were successful in the puncture (55%). During the 65 interventions, a total of five conceptional losses were observed, i.e., loss in 7.7% of the total number of interventions (n=65), 13.8% of the mares in which the puncture was successful (n=36) and 17% of the study population (n=29). The correlation between the age of the fetus and the possibility of performing or not performing the puncture showed that the higher the gestational age, the lower the possibility of performing the puncture (r = - 0.3877; P = 0.0005). In the analysis of the correlation between the age of the fetus and the incidence of abortion, a low correlation was found without significance (r = 0.1114; P=0.1888) and that the diameter of the needles is directly related to both the success of the puncture and the occurrence of abortion.
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