TECHNICAL AND ECONOMIC-FINANCIAL VIABILITY OF TILAPIA FARMING USING NATURAL FEED
DOI:
https://doi.org/10.56238/revgeov17n3-145Keywords:
Tilapia, Oreochromis niloticus, Macrophytes, SDG 2, Natural Feeding, Complex SystemsAbstract
Hunger and food insecurity persist as critical global challenges, exacerbated by socioeconomic inequalities, climate change, and increasing pressure on natural resources. According to the Food and Agriculture Organization of the United Nations (FAO, 2023), more than 735 million people still face some degree of hunger, highlighting the urgency of sustainable and inclusive productive systems. In this context, Sustainable Development Goal 2 (SDG 2) — “Zero Hunger and Sustainable Agriculture” — sets targets that include the eradication of hunger and the promotion of resilient agricultural practices. The search for innovative solutions for food production thus becomes a strategic priority. Aquaculture emerges as a promising alternative for sustainably expanding the supply of animal protein. Among farmed species, tilapia (Oreochromis niloticus) stands out as the most widely cultivated worldwide. Within this framework, the present study aims to demonstrate the technical and economic-financial feasibility of tilapia farming based exclusively on natural feeding, as an innovative and sustainable alternative aligned with the goals of SDG 2. A literature review was conducted to identify animal and plant species potentially usable as natural feed for tilapia. Based on this review, a mix of natural food sources was selected, composed of Hermetia illucens, Tenebrio molitor, Eichhornia crassipes, Lemna minor, Wolffia brasiliensis, and Phalloceros harpagos or Poecilia reticulata. The selection was based on criteria prioritizing ease of cultivation by laypersons with limited time availability. The project was submitted to the Ethics Committee on the Use of Animals (CEUA) of Univille and received authorization from ICMBio for its execution. The experiment was conducted on a small-scale rural property, using triplicate treatments and a control. Over a six-month period, four groups of 110 tilapia each were maintained in four 1,000-liter water tanks and fed twice daily—one group with commercial feed and three with the natural feeding mix. In addition to the food provided, consisting of larvae of Hermetia illucens and Tenebrio molitor, each tank contained a floating island built with garden hose and a wire frame covered with plastic mesh, where Eichhornia crassipes, Lemna minor, and Wolffia brasiliensis were cultivated, serving as habitat and protection for the reproduction of Phalloceros harpagos or Poecilia reticulata. Leaves, roots, and fish that extended beyond the mesh were continuously available as food for the tilapia. One of the challenges encountered, which affected tilapia development, was the low water temperature (average maximum of 26.6 °C and average minimum of 21.6 °C). Multiple areas of knowledge underpin the theoretical framework of this research, characterizing its interdisciplinary nature. Agronomy, Animal Science, Zoology, Botany, Fisheries Resources, and Fisheries Engineering converge to demonstrate that sustainable aquaculture production requires a systemic approach. The tested system was distinguished by the presence of multiple symbiotic relationships (Morin, 2011) and by being autopoietic (Maturana & Varela, 2006), thus constituting a complex adaptive system (Morin, 2011). The research was successful in demonstrating the technical and economic-financial feasibility of tilapia farming with natural feeding, showing that the proposed system can be applied both commercially and in subsistence initiatives. In the financial projection proposed in this study, with a one-year cultivation period, the operating result was on the order of 350%. In addition, the system proved to be an environmentally appropriate strategy for the use of organic waste. Another important aspect is that all components of the natural feeding system can also be directly commercialized. The experiment revealed numerous possibilities in the intended direction, demonstrating that the tilapia farming system based on natural feeding can be applied in different productive and social contexts. The results suggest that this approach contributes not only to the environmental and economic sustainability of aquaculture, but also to the promotion of food security and inclusion. The proposed model reinforces the importance of innovation based on ecological principles and the efficient management of available resources, aligning with the objectives of SDG 2 and with contemporary challenges in sustainable food production.
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References
Aboul-Enein, A. M., Lightfoot, D. A., Shalaby, E. A., El-Shemy, H. A., Zahran, M. M., & Shanab, S. M. M. (2014). Cytotoxic and antioxidant properties of active principals isolated from water hyacinth against four cancer cells lines. BMC Complementary and Alternative Medicine, 1472-6882. Springer Nature.
Abreu, M. G., Gonçalves, A. A., Alves, G., Barreto, N. S. E., Bentsen, H. B., Boari, C. A., Britto, E. N., Cyprian, O., Eknath, A. E., Esteves, E., Farias, M. C. A., Forsythe, S. J., Franco, B. D. G. M., Frazier, W. C., Gaspar Jr, J. C., Germano, P. M. L., Gonçalves, A. A., Jay, J. M., Soares, K. M. P., Lanzarin, M., Souza, L. B., Leitão, M. F. F., Machado, T. M., Massaguer, P. R., Nora, N. S., Nunes, M. L., Ordóñez, J. A., Rall, V. L. M., Santos, E. B., Simões, M. R., Soares, V. M., Soccoll, M. C. H., Souza, M. R. L., & Zanolo, R. (Eds.). Qualidade microbiológica de filés de tilápia do Nilo (Oreochromis niloticus) durante o armazenamento em gelo. Ciência Rural, ISSN 0103-8478, FapUNIFESP (SciELO).
Abro, Zewdu et al. 2020. Socio-economic and environmental implications of replacing conventional poultry feed with insect-based feed in Kenya. Journal of Cleaner Production, Elsevier BV
Hameed, A., Majeed, W., Naveed, M., Ramzan, U., Bordiga, M., Hameed, M., Ur Rehman, S., & Rana, N. (2022). Success of Aquaculture Industry with New Insights of Using Insects as Feed: A Review. Fishes, 7(6), 395. https://doi.org/10.3390/fishes7060395.
Appenroth KJ, Sree KS, Bog M, Ecker J, Seeliger C, Böhm V, Lorkowski S, Sommer K, Vetter W, Tolzin-Banasch K, Kirmse R, Leiterer M, Dawczynski C, Liebisch G, Jahreis G. (2018). Nutritional Value of the Duckweed Species of the Genus Wolffia (Lemnaceae) as Human Food. Front Chem. doi: 10.3389/fchem.2018.00483. PMID: 30420949; PMCID: PMC6215809.
Araújo Neves, J., Calvet, R. M., Carvalho Alves, V., Escorcio, R. E., Evangelista Lima, C., Pereyra, C. M., Sanches Muratori, M. C., & Teixeira de Oliveira Santo, J. (2015). In vitro adsorption of ochratoxin A by probiotics used in aquaculture. Acta Veterinaria Brasilica, ISSN 1981-5484, Editora da Universidade Federal Rural do Semi-Árido - EdUFERSA.
Arregi, Maite, Subinas, Beñat, Elissalt, Oier, Arrieta, Oihane, Oreja, Leire, Barriola Baraibar, Mari Jose, Saralegui Otamendi, Ainara (2021). Landare eta arrainen ekoizpen jasangarrirako akuaponia sistema baten garapena Tenebrio molitor intsektuaren larbak erabiliz. DOI: 10.1387/ekaia.21850, ISSN: 0214-9001.
Arya, V., Attermeyer, K., Fiener, P., Flury, S., Jayakumar, R., Premke, K., Steger, K., van Geldern, R., & Wilken, F. (2017). Invasive floating macrophytes reduce greenhouse gas emissions from a small tropical lake. Scientific Reports, 2045-2322. Springer Science and Business Media LLC.
Asadi, S., Leitão, A., Lymbery, A. J., Ringø, E., Shafiei, S., Soltani, M., & Van Doan, H. (2022). Streptococcosis a re-emerging disease in aquaculture: Significance and phytotherapy. Animals, 2076-2615. MDPI AG.
Barbosa, T. S., Cavaglieri, L. R., Dias, E. O., Keller, K. M., Oliveira, A. A., Pereyra, C. M., Rosa, C. A. da R., Silva, P. P. O., Soleiro, C. A. (2013). Mycobiota and mycotoxins present in finished fish feeds from farms in the Rio de Janeiro State, Brazil. International Aquatic Research, ISSN 2008-6970.
Bbosa, T. et al. (2020). Nutritional characteristics of selected insects in Uganda for use as alternative protein sources in food and feed. Journal of Insect Science, Oxford University Press (OUP)
Biasato, I. et al. (2019). Effect of dietary supplementation with insect fats on growth performance, digestive efficiency and health of rabbits. Journal of Animal Science and Biotechnology, Springer Science and Business Media LLC
Blanco Salas, J., Brufao Curiel, P., Ruiz Téllez, T., & Vázquez Pardo, F. M. (2016). Pasado, presente y futuro de una invasión biológica: Eichhornia crassipes (Mart.) Solms (camalote) en el río Guadiana. Conservación Vegetal, 1137-9952. Universidad Autónoma de Madrid. Departamento de Biología.
Borgemeister, C. et al. (2019). Influence of temperature on selected life-history traits of black soldier fly (Hermetia illucens) reared on two common urban organic waste streams in Kenya. Animals, MDPI AG.
Borges, A. M. (2009). Criação de tilápia, 2. ed. Brasília, DF: Emater-DF (Coleção Emater, ISSN 167 6-9279; n.18).
Boscolo, W. R., Bueno, G. W., Coldebella, A., Feiden, A., & Signor, A. A. (2010). Rações orgânicas suplementadas com farinha de resíduos de peixe para juvenis da tilápia do Nilo (Oreochromis niloticus). Revista Ciência Agronômica, ISSN 1806-6690, FapUNIFESP (SciELO).
Bruni, Leonardo et al. (2019). Effects of Graded Dietary Inclusion Level of Full-Fat Hermetia illucens Prepupae Meal in Practical Diets for Rainbow Trout (Oncorhynchus mykiss). Animals, MDPI AG.
Bruni, Leonardo et al. (2021). Appetite Regulation, Growth Performances and Fish Quality Are Modulated by Alternative Dietary Protein Ingredients in Gilthead Sea Bream (Sparus aurata) Culture. Animals, MDPI AG.
Camargo-Castellanos J.C., Flores-García L., Herrera-Díaz I.E., Álvarez-González C.A., Albertos-Alpuche P.J., Martínez-Yáñez R. (2022). System management of Lemna minor in aquaponics. Aquaculture Research, DOI: 10.1111/are.15637, ISSN: 1355557X.
Campeche, D. F. B., Cruz Neto, M. A. da, Melo, F. V. S. T. de, Melo, J. F. B., Moraes, G., & Souza, A. M. de. (2018). Replacing cornmeal with mango meal in diets for juvenile Tambaqui Colossoma macropomum: growth and metabolic parameters. Boletim do Instituto de Pesca, ISSN 1678-2305.
Cardenete Hernández, Gabriel et al. (2022). Fishmeal Dietary Replacement Up to 50%: A Comparative Study of Two Insect Meals for Rainbow Trout (Oncorhynchus mykiss). Animals, MDPI AG.
Chakrabarti R, Clark WD, Sharma JG, Goswami RK, Shrivastav AK, Tocher DR. (2018) Mass Production of Lemna minor and Its Amino Acid and Fatty Acid Profiles. Front Chem. doi: 10.3389/fchem.2018.00479. PMID: 30374437; PMCID: PMC6196230.
Chellappa, N. T., Chellappa, S., da Costa, S. A. G. L., da Silva, D. A., & Pessoa, E. K. R. (2012). Feeding ecology of Astyanax lacustris (Osteichthyes: Characidae) from Lagoa do Piató, Assu, Rio Grande do Norte, Brazil. Revista Biota Amazonia, ISSN 2179-5746.
Corrêa, R. de O., Silva, R. S. da. (2022). Bases para o manejo alimentar sustentável na piscicultura. Brasília, DF: Embrapa. ISBN 978-65-89957-16-4.
Costa, D. P. S., Trabuco, E., & Romanelli, P. F. (s.a.). Aproveitamento de vísceras não comestíveis de aves para elaboração de farinha de carne. Ciência e Tecnologia de Alimentos, ISSN 0101-2061. FapUNIFESP (SciELO).
Dediu L., Cristea V., Docan A. (2012). Bioremediation of recirculating systems effluents as a method to obtain high-quality aquaculture products. Journal of Environmental Protection and Ecology, ISSN: 13115065.
Dieleman J., Cocquyt C., Nyingi W.D., Verschuren D. (2021). Seasonality in diet and feeding habits of the endemic Chala tilapia (Oreochromis hunteri) and two introduced tilapiine cichlids in Lake Chala, East Africa. Hydrobiologia, DOI: 10.1007/s10750-020-04427-3, ISSN: 00188158.
El-Desouky, FF, Ibrahim, MA, Abd El-Razek, IM, El-Nabawy, EM, Amer, AA, Zaineldin, AI, Gewaily, MS, Dawood, MAO (2024). Improving Yellow Mealworm (Tenebrio molitor) Utilization with Sodium Butyrate in Nile Tilapia Diets: Effects on Growth Performance, Intestinal Histology, Antioxidative Response, and Blood Biomarkers. AQUACULTURE NUTRITION, ISSN: 1353-5773.
El-Naggar H.A., Khalaf Allah H.M.M., Masood M.F., Shaban W.M., Bashar M.A.E. (2019). Food and feeding habits of some Nile River fish and their relationship to the availability of natural food resources. Egyptian Journal of Aquatic Research, DOI: 10.1016/j.ejar.2019.08.004, ISSN: 16874285.
EMBRAPA. (2007). Produção de tilápia: Mercado, espécie, biologia e recria. Circular Técnica 45. Teresina, PI. ISSN 0104-7633.
Enyi, C. N., Uwakwe, A. A. & Wegwu, M. O. (2020). Nutritional Potentials of Water Hyacinth (Eichhornia crassipes). Direct Research Journal of Public Health and Environmental Technology. Vol. 5 (2), pp.14-18.
Fetahi T., Rothhaupt K.-O., Peeters F. (2018). Dietary map of Nile tilapia using stable isotopes in three tropical lakes, Ethiopia. Ecology of Freshwater Fish, DOI: 10.1111/eff.12361, ISSN: 09066691.
Fiordelmondo, E, Ceschin, S, Magi, GE, Mariotti, F, Iaffaldano, N, Galosi, L, Roncarati, A (2022). Effects of Partial Substitution of Conventional Protein Sources with Duckweed (Lemna minor) Meal in the Feeding of Rainbow Trout (Oncorhynchus mykiss) on Growth Performances and the Quality Product. PLANTS-BASEL, ISSN: 2223-7747.
Goyal, S, Ott, D, Liebscher, J, Hufling, D, Muller, A, Dautz, J, Gutzeit, HO, Schmidt, D, Reuss, R (2021). Sustainability Analysis of Fish Feed Derived from Aquatic Plant and Insect. SUSTAINABILITY.
Guevara Granja, M. F., & Ramírez Cando, L. J. (2015). Eichhornia crassipes, su invasividad y potencial fitorremediador. La Granja, ISSN: 1390-8596, 1390-3799.
Gülçin I., Kireçci E., Akkemik E., Topal F., Hisar O. (2010). Antioxidant, antibacterial, and anticandidal activities of an aquatic plant: Duckweed (Lemna minor L. Lemnaceae); [Bir su bitkisinin antioksidan, antibakteriyel ve antikandidal aktivitesi: Su mercimeǧi (Lemna minor L. Lemnaceae)]. Turkish Journal of Biology, DOI: 10.3906/biy-0806-7, ISSN: 13000152.
Hatab, Mahmoud H. (2019). Comparative Study of the Use of Insect Meal from Spodoptera littoralis and Bactrocera zonata for Feeding Japanese Quail Chicks. Animals, MDPI AG.
He J., Qiang J., Gabriel N.N., Xu P., Yang R. (2015). Effect of feeding-intensity stress on biochemical and hematological indices of GIFT tilapia (Oreochromis niloticus). Turkish Journal of Fisheries and Aquatic Sciences, DOI: 10.4194/1303-2712-v15_2_12, ISSN: 13032712.
Hempel, Arne-Jens et al. (2020). A comprehensive dynamic growth and development model of Hermetia illucens larvae. PLoS ONE, Public Library of Science (PLoS).
Hernandez, Carlos et al. (2021). Feeding live Black Soldier Fly larvae (Hermetia illucens) to laying hens: effects on feed consumption, hen health, hen behaviour and egg quality. Poultry Science.
Heuzé V., Tran G., Hassoun P., Régnier C., Bastianelli D., Lebas F., 2015. Water hyacinth (Eichhornia crassipes). Feedipedia, a programme by INRAE, CIRAD, AFZ and FAO. https://feedipedia.org/node/160 Last updated on October 13, 2015, 16:25
Hong J, Han T, Kim YY. (2020). Mealworm (Tenebrio molitor Larvae) as an Alternative Protein Source for Monogastric Animal: A Review. Animals (Basel). 0(11):2068. doi: 10.3390/ani10112068. PMID: 33171639; PMCID: PMC7695176.
Huis, Arnold van. (2016). Edible insects are the future? DOI: 10.1017/s0029665116000069, ISSN: 0029-6651, 1475-2719.
Islam, M. A., Mely, S. S., Alam, M. S., & Rahman, M. H. (2023). Effect of Different Feeding Rates on Growth Performance and Survival Rate of Tilapia (Oreochromis niloticus L. 1758) Fingerlings Reared in Rectangular Hapas. Asian Journal of Fisheries and Aquatic Research, 25(3), 18–30. https://doi.org/10.9734/ajfar/2023/v25i3664
Kasumyan A.O., Tinkova T.V. (2013). Taste attractiveness of different hydrobionts for roach Rutilus rutilus, bitterling Rhodeus sericeus amarus, and rainbow trout Oncorhynchus mykiss. Journal of Ichthyology, DOI: 10.1134/S0032945213040024, ISSN: 15556425.
Kroumov, A. D., Ross, A. A., Módenes, A. N., Souza, B. V., Geraldi, C. Q., Espinoza-Quiñones, F. R., & Dotto, J. (2013). Biosorption of BF-4B Reactive Red Dye by using Leaves of Macrophytes Eichhornia crassipes. Bulgarian Academy of Sciences, ISSN: 1314-1902, 1314-2321.
Lu, S., Taethaisong, N., Meethip, W., Surakhunthod, J., Sinpru, B., Sroichak, T., Archa, P., Thongpea, S., Paengkoum, S., Purba, R. A. P., & Paengkoum, P. (2022). Nutritional Composition of Black Soldier Fly Larvae (Hermetia illucens L.) and Its Potential Uses as Alternative Protein Sources in Animal Diets: A Review. Insects, 13(9), 831. https://doi.org/10.3390/insects13090831
Mahoney R., Weeks R., Huang Q., Dai W., Cao Y., Liu G., Guo Y., Chistyakov V.A., Ermakov A.M., Rudoy D., Bren A., Popov I., Chikindas M.L. (2021). Fermented Duckweed as a Potential Feed Additive with Poultry Beneficial Bacilli Probiotics. Probiotics and Antimicrobial Proteins, DOI: 10.1007/s12602-021-09794-4, ISSN: 18671306.
Mariana, E., Situmorang, A. H., & Yaman, M. A. (2021). Pengaruh Pemberian Konsentrat Fermentasi dan Silase Eceng Gondok (Eichhornia crassipes) terhadap
Maturana, H. (2006). Cognição, ciência e vida cotidiana. Belo Horizonte, MG: Editora UFMG.
Moakher, GV, Soltani, M, Shamsaee-Mehrjan, M, Kmali, A (2021). Effect of water hyacinth (Eichhornia crassipes) density on water quality, growth performance and survival of koi carp (Cyprinus carpio carpio) in an aquaponic system. IRANIAN JOURNAL OF FISHERIES SCIENCES, ISSN: 1562-2916.
Morin, E. (2011). O método 2: a vida da vida. Porto Alegre, RS: Sulina.
Narimbi J., Mazumder D., Sammut J. (2018). Stable isotope analysis to quantify contributions of supplementary feed in Nile Tilapia Oreochromis niloticus (GIFT strain) aquaculture. Aquaculture Research, DOI: 10.1111/are.13642, ISSN: 1355557X.
Nath K., Munilkumar S., Patel A.B., Kamilya D., Pandey P.K., Banerjee Sawant P. (2021). Lamellidens and Wolffia canopy improves growth, feed utilization and welfare of Labeo rohita (Hamilton,1822) in integrated multi-trophic freshwater aquaculture system. Aquaculture, DOI: 10.1016/j.aquaculture.2020.736207, ISSN: 00448486.
Oliveira, Elenise Gonçalves de; Santos, Francisco José de Seixas; Pereira, Alitiene Moura L.; Lima, Carolyny Batista. (2007). Produção de tilápia: Mercado, espécie, biologia e recria. Circular Técnica, Teresina-PI, EMBRAPA
ONU. (2021). Relatório anual 2021. Nações Unidas. Brasil
ONU. (2015). Transformando Nosso Mundo: A Agenda 2030 para o Desenvolvimento Sustentável. Nações Unidas.
Opiyo, MA, Muendo, P, Mbogo, K, Ngugi, CC, Charo-Karisa, H, Orina, P, Leschen, W, Glencross, BD, Tocher, DR (2022). Inclusion of duckweed (Lemna minor) in the diet improves flesh omega-3 long-chain polyunsaturated fatty acid profiles but not the growth of farmed Nile tilapia (Oreochromis niloticus). ANIMAL FEED SCIENCE AND TECHNOLOGY, DOI: SEP 2022, ISSN: 0377-8401.
Osti, JAS, do Carmo, CF, Cerqueira, MAS, Giamas, MTD, Peixoto, AC, Vaz-dos-Santos, AM, Mercante, CTJ (2020). Nitrogen and phosphorus removal from fish farming effluents using artificial floating islands colonized by Eichhornia crassipes. AQUACULTURE REPORTS, ISSN: 2352-5134.
PeixeBR. (2022). Anuário brasileiro da piscicultura Peixe BR 2022. São Paulo: Peixe BR Associação Brasileira de Piscicultura.
Pereira, D. L. N. C., Areco, K. C. N., Duarte, J. M., Shimaoka, A. M., Silva Junior, A. C. da ., Machado, V. T., Bandiera-Paiva, P., & Ancao, M. S. (2014). Tabela de Composição Química dos Alimentos - TABNUT (Versão 3). https://tabnut.dis.epm.br.
Peters, RR, Morales, ED, Morales, NM, Hernández, JL (2009). Feeding Quality Evaluation of Lemna obscura Meal as Ingredients in the Elaboration of Food for Red Tilapia (Orechromis spp.). REVISTA CIENTIFICA-FACULTAD DE CIENCIAS VETERINARIAS, ISSN: 0798-2259.
Pradhan A., Patel A.B., Singh S.K. (2019). Evaluation of live duckweed, Wolffia globosa as an allochthonous feed for Labeo rohita fry during nursery rearing. Aquaculture Research, DOI: 10.1111/are.14025, ISSN: 1355557X.
Queiroz, J. F. De; Alves, J. M. C.; Losekann, M. E.; Frasca-Scorvo, C. M. D.; Scorvo Filho, J. D.; Ferri, G. H.; Ishikawa, M. M. (2021). Manejo alimentar e da qualidade da água na produção de tilápia-do-nilo (Oreochromis niloticus). Jaguariúna, SP: Embrapa Meio Ambiente.
Reyes de Cabrales, C. E. (2009). Elaboración de abono orgánico a partir de plantas acuáticas: Elodea (Hydrilla verticillata) y Jacinto o Lirio de agua (Eichhornia crassipes), procedentes del Lago de Coatepeque y Lago de Güija. ITCA Editores, ISSN: 2070-0458.
Roy, K., Kajgrova, L., Capkova, L., Zabransky, L., Petraskova, E., Dvorak, P., Nahlik, V., Kuebutornye, F.K.A., Blabolil, P., Blaha, M., Vrba, J., and Mraz, J. (2024). Synergistic digestibility effect by planktonic natural food and habitat renders high digestion efficiency in agastric aquatic consumers. Science of the Total Environment, 927, 172105.
Said, I., Sangkota, V. D., & Supriadi, S. (2017). Pengaruh Aktivasi Kimia Arang Tanaman Eceng Gondok (Eichhornia Crassipes) terhadap Adsorpsi Logam Timbal (Pb). Tadulako University, ISSN: 2477-5185.
Sánchez-Muros M.J., de Haro C., Sanz A., Trenzado C.E., Villareces S., Barroso F.G. (2016). Nutritional evaluation of Tenebrio molitor meal as fishmeal substitute for tilapia (Oreochromis niloticus) diet. Aquaculture Nutrition, DOI: 10.1111/anu.12313, ISSN: 13535773.
SENAR. (2019). Piscicultura: alimentação. Coleção Senar, Brasília-DF, Senar.
Soares L.J.F., Ribeiro G.O., Freitas D.F., Madureira E.R., Aiura F.S., Maciel M.P., Nascimento J.C.S., Ribeiro A.G., Santos S.H.S., Costa D.V. (2023). Performance, blood and histological parameters and gene expression of lipogenic markers in tilapia (Oreochromis niloticus) fed Tenebrio molitor larvae meal. Journal of Insects as Food and Feed, DOI: 10.1163/23524588-20230063, ISSN: 23524588.
Sudarmadji, S., Sugiharto, E., Suprayogi, S., & Wantasen, S. (2012). Dampak Transformasi Nitrogen Terhadap Lingkungan Biotik Di Danau Tondano Provinsi Sulawesi Utara (The Impact of Nitrogen Transformation on the Biotic Environment in the Lake Tondano North Sulawesi). Jurnal Manusia dan Lingkungan, ISSN: 2460-5727, 0854-5510. Gadjah Mada University.
Sunil, K. R., John, M., Girisha, S. T., & Girish, V. (2015). A Comparative Study of Bioethanol Production from Aquatic Weeds. International Journal of Applied Sciences and Biotechnology, 2091-2609. Nepal Journals Online (JOL).
Tabela Brasileira de Composição de Alimentos (TBCA). Universidade de São Paulo (USP). (2023). Food Research Center (FoRC). Versão 7.2. São Paulo. Acesso em: 20 abr 2025. Disponível em: http://www.fcf.usp.br/tbca.
Temesgen M., Getahun A., Lemma B., Janssens G.P.J. (2022). Food and Feeding Biology of Nile Tilapia (Oreochromis niloticus) in Lake Langeno, Ethiopia. Sustainability (Switzerland), DOI: 10.3390/su14020974, ISSN: 20711050.
Tesfahun A., Alebachew S. (2023). Food and feeding habits of the Nile tilapia Oreochromis niloticus (Linnaeus, 1758) from Ribb reservoir, Lake Tana sub-basin, Ethiopia. Cogent Food and Agriculture, DOI: 10.1080/23311932.2023.2212457, ISSN: 23311932.
Trindade M.E.J., Cetra M., Jucá-Chagas R. (2010). Ichthyofauna of the Ribeirão Limoeiro, Cachoeira River Basin, BA; [Ictiofauna do Ribeirão Limoeiro, Bacia do Rio Cachoeira, BA]. Biota Neotropica, DOI: 10.1590/s1676-06032010000400015, ISSN: 16786424.
Tubin J.S.B., Paiano D., Hashimoto G.S.D.O., Furtado W.E., Martins M.L., Durigon E., Emerenciano M.G.C. (2020). Tenebrio molitor meal in diets for Nile tilapia juveniles reared in biofloc system. Aquaculture, DOI: 10.1016/j.aquaculture.2019.734763, ISSN: 00448486.
Turck D., Bohn T., Castenmiller J., De Henauw S., Hirsch-Ernst K.I., Maciuk A., Mangelsdorf I., McArdle H.J., Naska A., Pelaez C., Pentieva K., Siani A., Thies F., Tsabouri S., Vinceti M., Aguilera-Gómez M., Cubadda F., Frenzel T., Heinonen M., Prieto Maradona M., Marchelli R., Neuhäuser-Berthold M., Poulsen M., Schlatter J.R., van Loveren H., Kouloura E., Knutsen H.K. (2023). Safety of water lentil protein concentrate from a mixture of Lemna gibba and Lemna minor as a novel food pursuant to Regulation (EU) 2015/2283. EFSA Journal, DOI: 10.2903/j.efsa.2023.7903, ISSN: 18314732.
Velichkova K.N., Sirakov N.I. (2013). The usage of aquatic floating macrophytes (Lemna and Wolffia) as biofilter in recirculation aquaculture system (RAS). Turkish Journal of Fisheries and Aquatic Sciences, DOI: 10.4194/1303-2712-v13_1_13, ISSN: 13032712.
Vinogradskaya M.I., Kasumyan A.O. (2019). Palatability of Water Organisms for Nile Tilapia Oreochromis niloticus (Cichlidae). Journal of Ichthyology, DOI: 10.1134/S0032945219030196, ISSN: 00329452.
Wagaw S., Mengistou S., Getahun A. (2022). Diet composition and feeding habits of Oreochromis niloticus (Linnaeus, 1758) in Lake Shala, Ethiopia. Fisheries and Aquatic Sciences, DOI: 10.47853, ISSN: 22341749.
Xu S., Chen Z., Huang H., Huang X., Li S. (2010). Food sources of oreochromis niloticus in the mangrove and plantation-aquaculture ecological coupling systems. Zhongshan Daxue Xuebao/Acta Scientiarum Natralium Universitatis Sunyatseni, ISSN: 05296579.
Zhu J.J., Huang D.L., Fu Q., Ao Q.W., Tan Y., Lan G.Q., Guo Y.F., Zhang M., Gan X., Jiang H.S. (2015). Proteomic analysis of genetic improvement of farmed tilapia (GIFT) liver. Current Proteomics, DOI: 10.2174/157016461202150903115040, ISSN: 15701646.